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Paper Type | : | Research Paper |
Title | : | Review: Immobilization and application of lipase |
Country | : | China |
Authors | : | Naeema Matuoog || Yan Yunjun |
: | 10.9790/3008-1205010116 |
Abstract: The current demands of the world's biotechnological industries are enhancement in enzyme productivity and development of novel techniques for increasing their shelf life. Immobilized enzymes are more robust and more resistant to environmental changes than free enzymes in solution. Enzyme immobilization provides an excellent base for increasing availability of enzyme to the substrate with greater turnover over a considerable period of time. Several natural and synthetic supports have been assessed for their efficiency for enzyme immobilization..............
Keywords: Enzyme immobilization; Thermomyceslanuginosus lipase (TLL), carbon nanotube, magnetic nanoparticles, Applications.
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Acta,1958, 30(3): 513-521.
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micelles revealed by X-ray crystallography. Nature,1993, 362(6423): 814-820.
[4]. Verger R. ‗Interfacial activation'of lipases: facts and artifacts. Trends in Biotechnology,1997, 15(1): 32-38.
[5]. Ferrato F, Carriere F, Sarda L, et al. [16] A critical reevaluation of the phenomenon of interfacial activation. Methods
in enzymology,1997, 286: 327-347.
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Abstract: Kyllinga triceps rottb in commonly grown monocot grass of Gwalior Chambal region. In ayurvedic literatures it is known as musta. In various ethno botanical studies grass is reported to be diuretic, anti inflammatory hepatoprotective and antidiabetic. In present study we have been evaluated kullinga triceps rhizomes for its diuretic, nephroprotective and anti inflammatory potential.
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[3]. Amrita Bhaiji, Rakesh Sagar, Subhan Gankar Nandy, Ranjah Sudhanshu Swain, Bhusan Himanshu, Sahoo, International Research Journal of Pharmacy, 2012, 3, 5, 246-250.
[4]. Rajesh Kumar S., Manohar S., Ramesh K, International journal of pharm fech research, 2014, 6, 1, 403-408.
[5]. Patil S Praveen, Medabala tambi, chrstina, Suyana Bahu Suresh, Asian Journal of Pharmaceutical and clinical Research, 7, 4, 2014, 157-159.
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Abstract: This study was conducted for the period from 1/11/2015 to 15/6/2016 in Baquba city in Iraq. 74 samples were collected from burns with ten isolate (32.2%), wounds infections with six isolate (13..9%) from Baquba General Hospital. The results showed that 15 isolate (93.75%) were able production Lecithenase . Furthermore, 13(81.25%) isolate were able production Haemolysin,and 14 (87.5%) were able production proteinase .The sensitivily of these isolates were tested against (12) antibiotics. The results showed that the highest resistances were for Amoxicillin ,Ampicillin , Cefotaxime ,Trimethoprime with 100% , while the lowest resistance was for Ciprofloxacin 25% , while it showed high sensitivity toward combination Emipenem with 100% .As well as, 11 isolate(68.75%) were able to production β-lactamase., Furthermore, six isolate (37.5%) were able to production metalloβ-Lactamases. Finally, four isolate (25%) were able to production Extended Spectrumβ-Lactamases enzyme.............
Keywords: Pseudomonas aeruginosa , β-lactamases, metallo β-Lactamases , Extended Spectrumβ-Lactamases enzyme, Antibiotics
[1]. Karadzic I, Masui A, Zivkovic LI, Fujiwara N.(2006) Purification and characterization of an alkaline lipase from Pseudomonas
aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid. J Biosci Bioeng.; 102:82–89. [PubMed:
17027868].
[2]. Alammary , M. J.(2013) . Detection of some carbapenem-resistant genes of Pseudomonas aeruginosa isolates from Al-Hilla
teaching hospital . reserch College of Science. Babylon .Univ. (M. Sc. Thesis in Microbiology)
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sputa from patients with cystic fibrosis". Infect. Immun. 60(11):4687-4694. 1992 ).
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Abstract: Background: The great majority of individuals infected with the A (H1N1) virus experience uncomplicated influenza-like illness, with full recovery within 1 week, even without medical treatment, whereas a small subset of patients develops ever progressive disease. Number of Swine Flu Cases and Deaths in India in 2009 was 272 Cases and 981 death while in 2015 was 342 Cases & 2115 Death. The H1N1 virus outbreak had previously occurred India during the 2009 flu pandemic. The virus killed 981 people in 2009 and 1763 in 2010. The mortality decreased in 2011 to 75. It claimed 405 lives in 2012 and 699 lives in 2013. In 2014, a total of 218 people died from the H1N1 flu............
[1] www.mapsofindia.com/maps/mapinnews/swine-flu-india.html
[2] Swine flu outbreak: 743 deaths, 12,000 cases set alarm bells ringing". Hindustan Times. 21 February 2015. Retrieved 21 February 2015.
[3] Ministry of Health and Family Welfare, Government of India. Pandemic Influenza (H1N1)-Situational Update.
[4] Singh M et al Recent outbreak of Influenza A H1N1 (Swine Flu) in Western Rajasthan J Med Allied Sci 2013;3(2)
http://mohfw-h1n.nic.in/document/PDF/ (Last accessed on March 20, 2012).
[5] Sam IC, S Abu Bakar S. Pandemic Influenza A (H1N1) 2009 in Malaysia The Next Phase. Med J Malaysia 2009; 64: 105.
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Abstract: An ethnobotanical survey of plants associated with temple rituals and nurtured in the temple yards in the Luni sub district of Jodhpur carried out during 2015-16. The study documents 74 plant species of trees (54%), shrubs (34%), herbs (7%) and climbers (5%) conserved in studied temple yards of Luni sub district. These plants belonging to 70 genera and 37 families are protected and conserved in religious places, two plants Rohida (Tecomella undulata D. Don) and Ashoka (Saraca asoca (Roxb). Willd) are endangered and vulnerable plant species respectively according to IUCN (International Union for Conservation of Nature). The paper enumerates their local names, botanical names and life forms. Recorded economic and medicinal values of these plants are described.
Keywords: Worshipped plants, Ornamental plants, Medicinal plants, Economic importance.
[1] Badoni A, Badoni K. Ethno botanical Heritage. In: Kandari OP, Gusain OP, eds. Garhawal Himalaya; Nature, Culture & Society. Trans Media Srinagar (Garhwal) 2001; 125-47.
[2] Dhiman AK. Sacred Plants and their medicinal uses. Daya Publishing House, Delhi, 2003.
[3] Mohanty R, Tripathi BK, Panda T. Role of Temples and Other Holy Places in Plant Conservation of Odisha, India. International Journal of Conservation Science. 2012; 3(4) 301-308.
[4] Kumbhar A, Bharat, Dabgar J. To study of Aesthetic values of Some Traditional Worshipping Plants of Dang District. International Journal of Scientific Research April 2014; 3(4)46-47. [5] Martin GJ. Ethnobotany: A method manual. Chapman and Hall, London, 1995.
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Abstract: The morphological, anatomical, micro-morphological features and geographical distribution of Vaccaria Wolf. have been studied on plant samples collected from different northern and middle Iraqi districts where the plants usually grow, In general, the morphological results were similar with the description of the genus in Iraqi flora but with more details, the micro-morphological features of each seeds and pollen grain have been studied by SEM and gave a good distinguished characters. The anatomical studies of the roots, stems and leaves were carried out to obtain precise characteristics which are consider as original information about the genus in Iraq.
Keywords: Anatomy, Geographical distribution, Iraq, Morphology, Micro-morphology, Vaccaria
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[5] Sh. A. Ghazanfar, and J. R. Edmondson, Flora of Iraq, Vol. 5. Part 1 (Royal Botanic Garden, Kew, 2016).
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Paper Type | : | Research Paper |
Title | : | Incidence of Dengue in and around Kannur, Kerala |
Country | : | India |
Authors | : | Anuranjini c || Swathin Suresh || Dr.Lisha Lakshman |
: | 10.9790/3008-1205014650 |
Abstract: Dengue infection is an emerging disease and is a major health problem in our country. Globally the incidence of dengue has increased in the recent years. The WHO estimates that presently about two fifths of the world population is at risk for this viral infection. (2) Dengue fever (DF) is most common in older children, adolescents and adults. After an average intrinsic incubation period of 4–6 days (range 3–14 days), various non-specific, constitutional symptoms and headache, backache and general malaise may develop.(11)For transmission to occur the female Aedes aegypti must bite an infected human during the viraemic phase of the illness that manifests two days before the onset of fever and lasts 4–5 days after onset of fever. The present prevalence study is based on Dengue seropositive cases............
Keywords: Dengue, seropositive, Aedes aegypti, viraemic phase, Immunoglobulin M,
[1] 1.Scientific Working Group on Dengue. Report of the Scientific Working Group on Dengue, 1–5 October 2006. Geneva: World Health Organization; 2007.
[2] 2. Dengue haemorrhagic fever: diagnosis, treatment, prevention and control. 2nd edition. Geneva : World Health Organization. 1997
[3] 3.Behrens R., Carroll B. Dengue infections and travel. British Travel Health Association Newsletter: Travel wise 1999; 4. (Spring): pp. 4-5
[4] 4.Sarkar JK ., Chatterjee SN., Chakravarty SK., Haemorrhagic Fever in Calcutta: some epidemiological observations. Indian J Med Res 1964; 52: 651-9.
[5] 5.Chatterjee S N., Chakravarti SK., Mitra AC., Sarkar JK., Virological investigation of cases with neurological complications during the outbreak of haemorrhagic fever in Calcutta. J Indian Med Assoc 1965; 45: 314-6..
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Abstract: TFrom different hospitals in Baghdad city, 25 clinical isolates of Proteus spp. were collected from different clinical samples, all isolates were identified as Proteus mirabilis by using bacteriological and biochemical assays in addition to Vitek-2 identification system. 15 (60%) isolates were identifying as Proteus mirabilis. The susceptibility of P. mirabilis isolates towards cefotaxime and ceftazidime was (66.6 %), (20%) consecutively; while extended spectrum β-lactamases producing P. mirabilis percentage was (30.7 %). Because blaVEB-1 was documented as an important indicator for increasing risk of extended spectrum beta ß-lactamases producing P. mirabilis isolates...............
Keywords: Cephlosporine resistant, beta-lactamases, blaVEB-1 gene, EXBLs enzymes, Proteus mirabilis
[1]. Kocsis, B. and D. Szabó. Antibiotic resistance mechanisms in Enterobacteriaceae. Microbial pathogens and strategies for combating them: science, technology and education (A. Méndez-Vilas, Ed.).
[2]. Rozalski A.; A. Torzewska; M. Moryl; I. Kwil; A. Maszewska; K. Ostrowska; D. Drzewiecka; A. Zablotni; A. Palusiak; M. Siwinska and P. Staczek. 2012. Proteus sp. – an opportunistic bacterial pathogen – classification, swarming growth, clinical significance and virulence factors. Folia Biologica et Oecologica, 8: 1–17.
[3]. Pagani L.; R. Migliavacca; L. Pallecchi; C. Matti; E. Giacobone; G. Amicosante; E. Romero; and G. M. Rossolini. 2002. Emerging Extended-Spectrum -Lactamases in Proteus mirabilis. Journal of Clinical Microbiology, Apr. 2002, 40 (4):1549–1552.
[4]. Burton, G. R. W. and P. G. Engelkirk. 2004. Microbiology for the health sciences. 7th ed. Lippincott Williams and Wilkins. USA
[5]. Naas, T.; F. Benaoudia; S. Massurad and P. Nordmann. 2000. Integron- located VEB-1 extended- spectrum β-lactamase gene in a Proteus mirabilis clinical isoltes from Vietnam. Journal of Antimicrobial Chemotherapy, 46: 703-711..
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Paper Type | : | Research Paper |
Title | : | Histopathological study in the spleen of Albino Mice treated with glucosamine sulfate drug |
Country | : | Iraq |
Authors | : | Lamyaa .A. Fadhil |
: | 10.9790/3008-1205015864 |
Abstract: The current study designed to determine the effect of Glucosamine sulfate in the spleen organ of Albino mice .the study included (24) mice divided in to 3groups (control group had distilled water orally).The other groups treated with (1500, 2500) ml/kg .respectively for (2, 3) months .the spleen have been taken from dissected animal for microscopic preparation to study. Statistical results showed that the average weight of the spleen gradually decreased as the concentration and duration of the drug increased ,The histopathologicale changes appeared in the spleen of the exposure groups during (2,3) month ,the effects were Congestion,hyperplasia,atrophy, amyloid and necrosis.
Keywords: Glucosamine Sulfate, Spleen, Pathology, Amyloid, Hyperplasia, Atrophy
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[3]. Cerda, C.; Bruguera, M.and Parés ,A.( 2013). Hepatotoxicity associated with glucosamine and chondroitin sulfate in patients with chronic liver disease. World J Gas; 19(32): 5381-5384
[4]. Biggee, B.A.; Blinn ,C.M.; Nuite, M.; Silbert, J.E.and McAlindon, T.E.( 2007). Effects of oral glucosamine sulphate on serum glucose and insulin during an oral glucose tolerance test of subjects with osteoarthritis. Ann Rheum Dis.66:260-2.
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Abstract: Chronic kidney disease, a global health problem with possible complications and risk factors besides showing a remarkable negative effect in quality of life. The present study is concerned to evaluate the drugs used in management of Chronic Kidney Disease and to identify the side effects associated with treatment. A prospective observational study carried out in SVRR Govt. General Hospital during March to September 2014 (6months). A total of 65 patients were included in the study with the patient consent. A specially designed proforma was used to collect patient data. Prescriptions were...............
Keywords: Chronic kidney disease, contraindications, Pharmacokinetic profile, Narrow therapeutic index drugs
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[5]. Collins AJ, Foley RN, Chavers B, Gilbertson D, Herzog C, Johansen K, Kasiske B, Kutner N, Liu J, St Peter W, Guo H. 'United States Renal Data System 2011 Annual Data Report: Atlas of chronic kidney disease & end-stage renal disease in the United States. American journal of kidney diseases: the official journal of the National Kidney Foundation. 2012 Jan; 59(1 Suppl 1):A7-e1...
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Abstract: Degradation pathway of melphalan was validated by stability indicating reverse phase liquid chromatographic method. melphalan was subjected to stress condition using acid, base or by oxidation, and photolysis. Significant degradation was observed in acid and base stress conditions. The stress samples were assayed against a qualified reference standard and the mass balance was found close to 98.2 %. Efficient chromatographic separation was achieved on a Luna C18 (4.6 x 150 mm) 3 μm stationary phase with simple mobile phase combination. In the developed LC method, the..............
Keywords: Melphalan, method development, validation, precision and robustness
[1] United state pharmacopeia, USP 37 NF (40).
[2] British pharmacopeia, BP 2015.
[3] Indian Pharmacopeia.
[4] S.C. Sweetman, Martindale: The Complete Drug Reference, Pharmaceutical Press; 34th edition, 566 (2005).
[5] T. Facon, J.Y. Mary, and C. Hulin, Lancet, 370 (9594), 1209 (2007)...
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Abstract: Meloxicam (MX) is a nonsteroidal anti-inflammatory drug. Also, it is a more distinct COX-2 inhibitor than diclofenac with less secondary effects. Yet, its usage for the treatment of ophthalmic inflammations has retarded due to its low aqueous solubility and dissolution rate. The aim of this study was to prepare the inclusion complexes of MX with cyclodextrins to enhance its aqueous solubility, dissolution rate, and ocular bioavailability. Formulation of those complexes into different ocular delivery systems and also in-vitro and in-vivo assessments has not been accomplished in the earlier attempts. In this study, MX was complexed with each of hydroxyl propyl-β-cyclodextrin (HP-β-CyD) and β-cyclodextrin............
Keywords: cyclodextrins, inclusion complexes, meloxicam, ocular bioavailability
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Paper Type | : | Research Paper |
Title | : | Genetic Principles for Produce Plant: Potential, History,Methods & Goals |
Country | : | India |
Authors | : | Dr. P B Tiwary |
: | 10.9790/3008-12050197101 |
Abstract: Plant breeding uses genetics to generate healthier plants. This is done by selecting plants that are appealing or valuable commercially or artistically, managing their mating, and then selecting progeny. When done over many generations, such processes can change a plant population's genetic composition and value beyond what was naturally achievable. This article covers the history, methods, and ambitions of genetic principles to enhance plants, whereas heredity covers the biological components of plant breeding..
Keywords: Plant breeding methods, off springs, genetic principles ,hereditary compositions etc...
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