Abstract: Background: Basil (Ocimum sanctum) is a member of Lamiaceae family which is known as a medicinal plant. This research aims to determine the toxicity (LC50) of basil leaves. The extraction was carried out by maceration using methanol as a solvent. Toxicity test using the Brine Shrimp Lethality Test method. In this research there were 4 concentration i.e 50 ppm, 100 ppm, 300 ppm, 500 ppm and control, each of which was repeated three times. Each concentration used 10 Artemia salina larvae and the mortality of larvae was calculated after 24 hours. The calculation of the LC50 value was identified by the percentage of shrimp larvae mortality using probit analysis. The results showed that the LC50 value of the methanol extract of basil leaves was 53.6630 ppm. The extract is toxic if the LC50 value is < 1000 ppm. This indicates that the methanol extract of basil leaves is toxic and has the potential as an anti-cancer compound.
Key Word: Basil (Ocimum sanctum) Leaf; Toxicity; Brine Shrimp Lethality Test; LC50
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