Version-1 (May-June 2015)
Version 1 Version 2 Version 3 Version 4
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | "Comparative Antimicrobial Study of Cordia macleodii Hook" |
| Country | : | India |
| Authors | : | Dr.Om Narayan Chaubey || Dr. Ravi Upadhyay || Niraj Kumar Tripathi || Amulya Ranjan |
Abstract: This study was carried out with an objective to investigate the antibacterial and antifungal potential of Bark and leaves of Cordia macleodii Hook. Antibacterial activity of Ethanolic extracts of the Bark and leaves was carried out against four bacteria – Escherichia coli, Bacillus subtilis, Comamonas testosteroni and Pseudomonas plecoglossicida. The antifungal activity of the extracts was evaluated on three common pathogenic fungi Aspergillus flavus, Aspergillus niger and Candida albicans. The testing was done by the agar plate method. Zones of inhibition of extracts were compared with that of different standard like Ciprofloxacin for antibacterial activity and fluconazole for antifungal activity. The extracts showed antibacterial and antifungal activities comparable with that of standard against the organisms tested. Only bark extract of Cordia macleodii Hook showed positive results for two bacteria Comamonas testosteroni and Pseudomonas plecoglossicida out of all tested. The percentage inhibition value were found to be in range of 6mm to 9mm aginst Pseudomonas plecoglossicida and 7mm at the concentration of 100mg/ml of extract against Comamonas testosteroni but the other two concentration 50 and 25mg/ml showed no effect. The results showed that the inhibition of the bacterial growth was more pronounced on Pseudomonas plecoglossicida and Comamonas testosteroni as compared to the other tested organisms. The extract did not show the antifungal activity against all tested fungus.
Ke words: Antibacterial, antifungal, Cordia macleodii Hook.
[1]. Bendre A. Practical Botany, Meerut Rastogi Publication; 2007. pp. 8–11.
[2]. Qureshi NN, Kuchekar BS, Logade NA, Haleem MA, "Investigation of antimicrobial activity of Cordia macleodii and Leucas ciliata leaves " International Journal of PharmTech Research, 2 (1), 2010a: 118-120.
[3]. Bhide B , Acharya RN, Naria P, Pillai APG, Shukla VJ, "Pharmacognostic Evaluation of Cordia macleodii Hook. Stem Bark" 3 (26); 2011a: 49-53.
[4]. Bhide B , Pillai APG, Shukla VJ, Acharya RN, "Pharmacognostic evaluation of leaf of Cordia macleodiiHook., An ethnomedicinally important plant" Ayu, 2011 32(2): 254–257.
[5]. Qureshi NN, Kuchekar BS, Logade NA, Haleem MA, "Antioxidant and hepatoprotective activity of Cordia macleodiileaves" Saudi Pharm J. 2009b; 17(4): 299–302.
[6]. Sharma A, Acharya RN, Shukla VJ, Harisha CR, Gupta SK, " Identification of Hook through DNA finger Printing and stomatal index" Univerisal Journal of Pharmacy, 2013, 2 (3):98-101
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Rare Complication Following The Bite Of A Russell Viper-A Case Study |
| Country | : | India |
| Authors | : | Dr. Tanushree Mondal || Dr. Sandip Ghosh || Dr. Ritwika Mazumder |
Abstract: Envenomation resulting from snake bite is an important public health problem in tropical countries like Southeast Asia and throughout Indian subcontinent. Snake bite is associated with myriad of complications that can be life threatening .We hereby report a 23 year old countryman who was bitten by Russell's viper and was treated with antivenom and other medications. Although he recovered from the acute effects, he remained feeling unwell and was diagnosed one month later with hypopituitarism, an unusual complication of endocrine system. Replacement started with essential hormones such as hydrocortisone and Levo-Thyroxine. Keywords: Hypopituitarism, Russell's viper.
[1]. Antonypillai CN, Wass JA, Warrell DA, Rajaratnam HN, Hypopituitarism following envenoming by Russell's Vipers (Daboia siamensis and D. russelii )resembling Sheehan's syndrome:first case report from Sri Lanka , a review of the literature and recommendations for endocrine management. QJM2011 Feb;104(2):97-108.
[2]. Qnose H, Tamura Y,Fujita H, Nakano T, Shibasahi T. A case of Sheehan's Syndrome with panhypopituitarism due to the impairment of both the hypothalamus and pituitary. Endo.J 2003Aug;50(4),415-419.
[3]. Dhamoal DK, Das AK. Hypopituitarism following snake bite. J Assoc.Physician India 1998;(46):322.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Formulation and Evaluation of Benzyl Benzoate Emulgel |
| Country | : | Malaysia |
| Authors | : | Khuriah Abdul Hamid || Salizatul Ilyana Ibrahim || Muhammad Azril Hashim || Mohamed Salama |
Abstract: The aim of the following research was to formulate benzyl benzoate in emulgel form and to evaluate the release of the medicament from it. Emulgel containing 25% w/w of benzyl benzoate was prepared by addition of a gelling agent; Carbopol 934 to an emulsion using Span 80 as emulsifying agent. The release of benzyl benzoate was determined by Franz diffusion cell using cellulose acetate membrane for a period of five hours at 32°C. Benzyl benzoate was analyzed by ultra-violet (UV) Spectrophotometer at 256 nm. The prepared formula of benzyl benzoate emulgel proved to be more efficient than other externally applied topical preparations as lotion, ointment and gel.
Keywords: benzyl benzoate; Carpopol 934; emulgel; Franz diffusion cell
[1]. Prajapati M., Patel M., Patel K., Patel N.. Emulgels: A novel approach to topical drug delivery. International Journal of Universal Pharmacy and Bio Sciences 2(1) (2013),pp.134-148. [2]. Sngla,V., Sani,S., JosephI,B. and Rana,A.C. Emulgel: A new platform for topical drug delivery. International Journal of Pharma and Bio Sciences 3 (2012),pp.485-498.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Effect of an insecticide "Ekalux" on the Haematology of the Freshwater fish, "Labeorohita" |
| Country | : | India |
| Authors | : | K. Vijaya kumar || S. Binu kumari || M.Mohan kumar |
Abstract: In developing countries, an extensive use of insecticides to meet with increased agricultural needs is inevitable and the indiscriminate use and misuse of insecticides results in acute poisoning of the biosystems. The sublethal toxicity of the insecticide Ekalux on the fingerlings of Labeo rohita was evaluated to determine its effect on the haematological values. The acute and sublethal toxicity of the insecticide "Ekalux" on the fingerlings of Labeo rohita was evaluated to determine its effect on the haematological values. The fish was exposed to varying levels of the toxicant concentrations using static bioassay to determine the median lethal concentration.
[1]. Begum,G. 2004. Carbofuran insecticide induced biochemical alterations in liver and muscle tissues of the fishClariasbatrachus and recovery response. Aquatic Toxicol. 66: 83-92.
[2]. Suvetha, L., Ramesh,M. and Saravanan,M. 2010. Influence of Cypermethrin toxicity on ionic regulation and gill Na+/ K+ ATP are activity of a fresh water teleost fish, Cyprinuscarpio. Environ.Toxicol.Pharmacol., 29(1): 44-49.
[3]. T.Sahli, 1962 .Text book of clinical pathology,(Ed.Scward,Eimiller).Williams and Williams and Co., Baltimore, pp 35.
[4]. S.L.Shah, and A.Altindag,Haematological parameters of tench (Tincatinca L.) after acute and chronic exposure to lethal and sublethal mercury treatments. Bull.Environ.Contam.Toxicol, 73, 2004, 911-918.
[5]. D.Anderson, and G.W.Klontz, Basichaematology for the fish culturist,Ann.Northwest Fish Culture Conference , 16, 1965, 38-41.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Studies on the Physiological & Nutritional Effects of Newly Developed Low Cost Supplementary Formula Containing Less Familiar Food Stuffs on Experimental Animals |
| Country | : | India |
| Authors | : | Soma Chakrabarty || Santa Datta (De) |
Abstract: An attempt had been made to develop a low cost Ready to UseSupplementary Formula (RUSF) using kitchen waste such as jackfruit seed, watermelon seed, drumstick leaves and fish bone for lower income group of people in India. The objective of this research work is to check the physiological effect of RUSF on albino rats. Eighteen male Wister albino rats having body weight from 70-80 gm were divided into control and treated group. Control group was fed on stock diet and treated group was given RUSF for 28 days. Overall growth pattern, serum glucose, protein, calcium, liver glycogen and liver protein levels were almost similar in both groups without any significant difference. No significant difference was found in serum SGPT, SGOT and ALP levels, indicating normal liver function in both groups
[1]. Press note on poverty estimates.(2011-2012).Planning commission,Govt.of India,July 2013
[2]. Tananuwong K, Tulyathan V, Songjinda P, Jaiboon N.(2002). Some physico-chemicalproperties of jackfruit seed flour an starch. Sc. Asia 28 : 37-41.
[3]. Chakraborty P, Bhattacharyya DK, Bandyopadhyay NR, Ghosh M.(2013). Study on utilization of Jackfruit seed flour and de-fatted soy flour mix in preparation of breakfast cereal by twin-screw extrusion technology. Discovery 4(11):32-37.
[4]. El-Adawy TA, Taha KM.(2001).Characteristics and Composition of Watermelon, Pumpkin, and Paprika Seed Oils and Flours. J. Agric. Food Chem 49:1253−1259
[5]. Joshi P, Mehta D.(2010). Effect of dehydration on the nutritive value of drumstick leaves. Journal of Metabolomics and Systems Biology 1(1):5 -9.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | A revive of α-latrotoxin |
| Country | : | India |
| Authors | : | Ankit Kumar Jain || Dr. N.K. Dhakad || Dr. Vipulkeerti Sharma || Sachin Rahangdale |
Abstract: α-Latrotoxin is a black widow spider (Latrodectus mactans) venom toxin causing massive neurotransmitter release. Venomous animals use a highly complex cocktails of proteins, peptides and small molecules to subdue and kill their prey. Structure–function studies of spider toxins are leading to the discovery of novel therapeutic routes for neuromuscular diseases, pain and to a variety of other pathological conditions.
[1]. Adam-Vizi V, Deri Z, Bors P, et al (1993) Lack of involvement of [Ca2+]i in the external Ca2+- independent release of acetylcholine evoked by veratridine ouabain and α-latrotoxin possible role of [Na+]i. J Physiol Paris 87: 43–50.
[2]. Ashton AC, Rahman MA, Volynski KE, et al (2000) Tetramerisation of α-latrotoxin by divalent cations is responsible for toxin-induced non-vesicular release and contributes to the Ca2+-dependent vesicular exocytosis from synaptosomes. Biochimie 82: 453–468.
[3]. Ashton AC, Volynski KE, Lelianova VG, et al (2001) α-Latrotoxin, acting via two Ca2+-dependent pathways triggers exocytosis of two pools of synaptic vesicles. J Biol Chem 276: 44695–44703.
[4]. Auger C, Marty A (1997) Heterogeneity of functional synaptic parameters among single release sites. Neuron 19: 139–150.
[5]. Boehm S, Huck S (1998) Presynaptic inhibition by concanavalin A are α-latrotoxin receptors involved in action potential-dependent transmitter release. J Neurochem 71: 2421–2430.
[6]. Bogen E, Loomis R (1936) Poisoning poisonous spiders an experimental investigation in the control of the black widow spider (Latrodectus mactans). Cal West Med 45: 31–38.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | The effects of polybasic acid type on kinetics of the preparation of cottonseed oil based alkyd resins |
| Country | : | Nigeria |
| Authors | : | Ibanga O. Isaac || Okon D. Ekpa || Udofot J. Ekpe || Anduang O. Odiongenyi |
Abstract: The kinetics of the alcoholysis-polycondensation of cottonseed oil (COSO), glycerol (GL), phthalic anhydride (PA), Maleic anhydride (MA) and Succinic acid (SA) leading to the formation of alkyd resins were investigated. Three grades of alkyd samples formulated to have oil content of 50% were prepared with COSO, GL, PA (I), MA (II) and SA (III) respectively. The extent of polycondensation was monitored by measuring the volume of water of esterification evolved, determining the pH and acid value of aliquots of the reaction mixture at various intervals of time.
[1]. Patel, V. C., Varughese, J., Krishnamoorthy, P. A., Jain, R. C., Singh, A. K. and Ramamoorty, M. Synthesis of alkyd resin from Jatropha and rapeseed oils and their applications in electrical insulation. Journal of Applied Polymer Science, 78, 2008, 1826 – 1832.
[2]. Ling, J. S., Mohammed, I. A., Ghazali, A. and Khairuddean, M. Novel poly(alkyd-urethane)s from vegetable oils: Synthesis and properties. Industrial Crops & Products, 52, 2014, 74 – 84.
[3]. Alam, M., Akram, D., Sharmin, E., Zafar, F, F. and Ahmad, S. Vegetable oil based eco- friendly coating materials: A review article. Arabian Journal of Chemistry, 2014, (in press).
[4]. Shabeer, A., Sundararaman, S., Chandrashekhara, K. and Dharani, L. R. Physicochemical properties and fracture behaviour of soy-based resin. Journal of Applied Polymer Science, 105, 2007, 656 – 663.
[5]. Aydin, S., Akcay, H., Ozkan, E., Gunner, F. S. and Erciyes, A. T. The effects of anhydride type and amount on viscosity and film properties of alkyd resin. Progress in Organic Coating, 51, 2004, 273 – 279.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Study the pathogenesis of Candida albicans in animal model |
| Country | : | Saudi Arabia |
| Authors | : | H.S. Naher || N.N.Al-dabagh |
Abstract: The aim of this study was to investigate the effect Sereny test caused by Candida albicans in experimental induced ulcer in rabbit.Corneal ulcer was induced by superficially inoculated with 100μl of C. albicans containing1.5×108 CFU and dropped on left eye with sterilized PBS was topically applied to right eye in 50 rabbits .These animals were divided equally into two groups .The first group with nontreated with cyclophosphamide andsecondgrouptreatedwith cyclophosphamide (immunosuppressed) Seven days later corneas photographed and animals killed . After sacrificing the corneas of animals underwent routine histopathological examination on the day 1st, 3rd,7th days after inoculation.
[1]. Braun BR, Kadosh D, &Johnson AD (2001). NRG1, a repressor of filamentous Growth in C.albicans is down – regulated during filament induction . BMBOJ20:4753 – 61.
[2]. De Bernardis F, Chiani P, & Ciccozzi M, (1996). Elevated aspartic proteinase Secretion experimental pathogencity of Candida albicans isolates from oral cavities cavities of subjects infected with human immunodeficiency virus Infect Immun , 64 : 466 -471.
[3]. De Bernardis F, Muhlschlegel FA, Cassone A, &Fonzi WA. (1998) .The pH of the Host niche controls the gene expression in and virulence of Candida albicansInfect Immun 66:3317–3325 .
[4]. Gale CA, Bendle CM,& McClellan M (1998). Linkage of adhesion ,filamentatous growth and virulence in Candidas to a single gene INT.Science , 33 : 1355- 1358.
[5]. Hanslmaver & Roll (1978).Light and electron – microscopic examination of deep keratomycosis asperogillosis . Arch klin Exp Ophthalmol , 206 : 203-216.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Determination of Al, Ca, Cl, Cr, K, Mg, Sb and Ti in industrialized and formulated antihypertensive drugs using Neutron Activation Analysis |
| Country | : | Brazil |
| Authors | : | Josianne N. Silveira || Maria A. C. B. Menezes || Paulo. C. P. Lara || Mark. A. Beinner || José Bento B. da Silva |
Abstract: This work evaluated Al, Ca, Cl, Cr, K, Mg, Sb and Ti levels in capsules and tablets of the formulated and industrialised antihypertensive enalapril maleate and amlodipine besylate drugs used systematically. The neutron activation analysis was applied using the k0-standardization method. The accuracy was checked with certified materials. The detection limits ranged from 0.01 μg capsule-1 for Cr and Sb and 0.01 μg tablet-1 for Mg, Sb and Ti, and 400 μg capsule-1 to 1000 μg tablet-1 for Ca. In the 63 analysed samples, the element levels ranged from 0.064±0.005 μg tablet-1 for Sb and 36.2±4.5 mg tablet-1 for Ca. In formulated capsules, the levels varied between 0.010±0.001 μg capsule-1 for Sb to 30.5±1.1 mg capsule-1 for Mg.
Keywords: Inorganic constituents, industrialized and formulated antihypertensive drugs, Neutron Activated Analysis
[1]. A. Taylor, S. Branch, M. P. Day, M. Patriarca, M. White, Atomic spectrometric update. Clinical and biological materials, foods and beverages, J. Anal. At. Spectrom. 26(4), 2011, 653-692.
[2]. http://www.ib.unicamp.br/dep_biologia_animal/BE310, Principais contaminantes ambientais presentes em medicamentos e seus grupos farmacológicos, V. C. Flório. (Acessed in 08/04/2015).
[3]. A. G. de Oliveira, M. V. Scarpa, 2005. www.farmaceuticogaucho.pro.br/altercao.doc, (Acessed in 08/04/2015)
[4]. R.C. Rowe, P. J. Sheskey, M. E. Quinn, Handbook of Pharmaceutical Excipients (RPS Publishing, London, UK, 2009).
[5]. E. M. M. Flores, C. F. Bittencourt, L. V. Laporta, J. S. Barin, Controle de Metais Pesados em Produtos Farmacêuticos: Aspectos Analíticos Relevantes, Pharm. Technol. 4(1), 2000, 9-13.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Hydrocarbons, fatty acids and biological activity of date palm pollen (phoenix dactylifera L.) growing in Egypt |
| Country | : | Egypt |
| Authors | : | Mohamed H. M. Abed El-Azim || Fathy A. Yassin || Salam A. Khalil || Amani M. D. El-mesalamy |
Abstract: In this study, thenon-polar light petroleum ether anddiethyl ether extractsof date palm (Phoenixdactylifera L.)Pollen wereanalyzed by Gas chromatography–mass spectroscopy (GC-MS) and found to be rich withlipids and steroids. Fatty acids include Palmitic acid 38.52% and Hydnocarpic acid 44.23% while α-Sitosterol was the most prevalent Phytosterol14.40%. Un-saponifiable fraction and saponifiable fraction of Pet. ether and DEE extracts were screened for their in vitro antimicrobial activities against six bacterial species (Escherichia coli, Klebsiella species, Staphylococcus epidermidis, Bacillus cereus, Micrococcus luteus and Staphylococcus aureus).
[1] M.M. Abde-El-Mageed,Supplementation of snack food with pollen grains of date palm. Egyptian J. Food Sci, 15, 1987, 25-7.
[2] W. Al-Shahib, The fruit of the date palm: Its possible use as the best food for the future,International Journal of Food Science and Nutrition, 54, 2003,247−259.
[3] A. Zaid, P.F. De Wet, Botanical and Systematic Description of The Date Palm, Date Production Support Programmed, Date Palm Biotechnology, 114, 2002, 969-979.
[4] R.D. Bennet, E. Heftmann, Isolation of estrone and cholesterol from the date palm Phoenix Dactylifera. Photochemistry, 5, 1966, 231-235.
[5] F. Biglari, A. F. M. AlKarkhi,M. E. Azhar, Antioxidant activity and phenolic content of various date palms (Phoenix dactylifera) fruits from Iran,Food Chemistry, 107, 2008, 1636–1641.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | A Computational Strategy for Dose Adaptation at the Population and Group Levels |
| Country | : | Canada |
| Authors | : | Guillaume Bonnefois || Olivier Barrière || Fahima Nekka || Jun Li |
Abstract: With the intention to achieve the best therapeutic outcomes, dose adaptation underpins the clinical practice by tailoring dose and time in order to maximize efficacy while minimizing toxicity. Depending on the drug properties and the clinical context, three successive levels of dose adaptation can be considered, i.e., approaches based on population, group and individual. To make a rational choice for the dose adaptation level and determine the best drug regimens, we here propose a modeling and simulation strategy based on the platform provided by the population pharmacokinetic/pharmacodynamic methodology at population and group levels. In order to compare the performance of different dose and time schedules, we introduced probabilistic conceptualized time- and concentration-based therapeutic indicators.
[1]. P. Canal, E. Gamelin, G. Vassal, and J. Robert, "Benefits of pharmacological knowledge in the design and monitoring of cancer chemotherapy," PatholOncolRes, vol. 4, no. 3, pp. 171–8,1998.
[2]. S. L. Preston, "The importance of appropriate antimicrobial dosing: pharmacokinetic and pharmacodynamic considerations," Ann Pharmacother, vol. 38, no. 9 Suppl, pp. S14–8, Sep 2004
[3]. J. Kang, Y.-S.Park, S.-H.Kim, S.-H.Kim, and M.-Y. Jun, "Modern methods for analysis of antiepileptic drugs in the biological fluids for pharmacokinetics, bioequivalence and therapeutic drug monitoring," Korean J PhysiolPharmacol, vol. 15, no. 2, pp. 67–81, Apr 2011.
[4]. J. E. Ray, A. M. Keogh, A. J. McLachlan, and F. Akhlaghi, "Cyclosporin c(2) and c(0) concentration monitoring in stable, long term heart transplant recipients receiving metabolic inhibitors," J Heart Lung Transplant, vol. 22, no. 7, pp. 715–22, Jul 2003.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Crude Protein Profiling of Varieties of Capsicum annuum and Capsicum frutescens using SDS-PAGE. |
| Country | : | Nigeria |
| Authors | : | Olatunji T.L. || Morakinyo J.A. |
Abstract: The study was aimed at assessing the genetic variation and relatedness among four Capsicum varieties through electrophoretic separation of their leaf and seed proteins by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Total seed and leaf proteins were extracted and separated on 12% polyacrylamide gels using standard protocols. Distinct polymorphism in electrophoretic banding patterns of seed and leaf proteins was observed in the four Capsicum varieties through a total of thirty-eight (38) polypeptide bands in the seeds and a total of seventeen (17) polypeptide bands in the leaves analyzed. Variation existed not only in the number of bands but also in the intensity of bands in both the leaf and seed samples studied. Sokal and Sneath's coefficient of similarity revealed a generally high level of similarity in the seed protein bands of the four varieties studied ranging from 50% to 100%.
[1]. Aguegia, A., Fatoku, C.A. and Hahn, S.K. (1994). Leaf protein analysis of ten cocoyam (Xanthosoma sagitifolium (L.) Schott and Colocasia esculenta (L) Schott genotypes. Proc of the Fifth Symposium ISTRC-AB, 348-353 p.
[2]. Al-Wadi, M.H (2007). Palynological and cytological characters of three species of genus Solanum from Saudi Arabia. Journal of Biological Sciences, 7(4): 626-631.
[3]. Anu, A. and Peter, K.V. (2003). Analysis of seed protein of 29 lines of Capsicum annuum L. by polyacrylamide gel electrophoresis. Genetic Resources and Crop Evolution, 50:239-243.
[4]. Badr, A. (1995). Electrophoretic studies of seed proteins relation to chromosomal criteria and relationships of some taxa of Trifolium. Taxon 44: 183–191.
[5]. Bosland, P.W and Votava, E.J. (2000). Peppers: Vegetable and Spice Capsicums. CABI publishing, Wallingford. Pp. 204.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Efficacy of Neem Seed and Neem Seed Kernel Powders onthe Survival, Longevity and Fecundity of BlowflyChrysomya chloropyga (Wied.) (Diptera:Calliphoridae). |
| Country | : | Nigeria |
| Authors | : | A.A. Ajayi || W. A. Muse |
Abstract: First, second and third instar larvae of the blowfly, Chysomya chloropyga collected from laboratory stock were exposed to a mixture of ground rice and fish supplemented with 1, 2, 3, 4, 6 and 10 % concentrations of neem seed (NS) and neem seed kernel(NSK) powders respectively.Twenty pairs of laboratory-reared adult males and females were also maintained separately on diets treated with 1, 2, 4, 6 and 10% NS and NSK for survival, longevity, fecundity and progressive weights of adult male and female at 5-day interval.
[1]. Al-Fifi, Z. I. (2006): Molting inhibitory and lethal effects of azadirachtin on the desert locust,Schistocercagregaria (Forskal). Journal of Entomology,3(4): 312 - 318.
[2]. Al-Fifi, Z. I. (2009): Effect of different neem products on the mortality and fitness of adult Schistocercagregaria. Journal of King Abdulaziz University: Science, 21(2): 299 - 315.
[3]. Amirmohammadi, F.;Sendi, J. J. and Zibaee, A. (2012): Biomonitoring of the genotoxic and oxidative the effect of neem on mortality and physiological indices ofHyphantriacunea(Drury) (Lepidoptera). Munis Entomology & Zoology, 7 (1): 489 - 495.
[4]. Anantiko, L.; Banditising, G. and Ketavan, C. (1982): Joint FAO / IAEA information circular on radiation technique and their application to insect pests. No. 30, p. 9, abstract 4.
[5]. Anderson, G. S. (2001): Succession on carrion and its relationship to determining time of death. In Forensic Entomology: the utility of arthropods in legal investigations. J. H. Byrd, J. L. Castner (eds.), Boca Raton, CRC, pp. 143 – 175.
- Citation
- Abstract
- Reference
- Full PDF
| Paper Type | : | Research Paper |
| Title | : | Evaluation of Genetic Fidelity of in-Vitro propagated Decalepis hamiltonii Wight & Arn. using DNA Based Marker |
| Country | : | India |
| Authors | : | Pranay Kumar || Raviraja Shetty. G || Souravi. K || Rajasekharan. P.E |
Abstract: In-vitro culture stress might result in breakdown of control mechanisms causing instability of the genome in tissue cultured plantlets and hence these plantlets have to be subjected to assessment of genetic fidelity. We have used a DNA based molecular marker to assess the genetic stability of in - vitro regenerated Decalepis hamiltonii. The nodal explant responded satisfactory in terms of growth related traits when inoculated in the MS medium supplemented with BA(0.886mg/l)+2ip(0.24mg/l). When screened with 12 Random Amplified Polymorphic DNA (RAPD) primers, it produced clear reproducible and scorable bands. All banding profiles from micropropagated plants were monomorphic and similar to that of the mother plant. This study is of high significance as these could be commercially utilized for large scale production of true-to-type plantlets in D. hamiltonii.
Key words: Decalepis hamiltonii, in-vitro propagation, genetic fidelity, RAPD marker.
[1]. Borthakur M, Hazarika J, Singh RS. A protocol for micropropagation of A.galanga. Plant Cell, Tissue and Organ Culture. 1999. 55: 231-233.
[2]. Dey D, Das M, Sharma AK. Pharmacognosy of Indigenous Drugs. Central Council for Research in Ayurveda and Shiddha. New Delhi. 1999. 897.
[3]. Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull. 1990. 19: 11-15.
[4]. Gamble JS, Fisher CEC. Flora of the Presidency of Madras, Adlard & Aon Ltd. London. 1957. volume-2.
[5]. George J, Ravishankar GA, Perira J, Divakar S. Bioinsecticide from swallow root (Dacalepis hamiltonii Wight and Arn.) protects food grains against insect infestation. Current Science. 1999. 77: 501-502.
[6]. Jadhav SN, Reddy KN. In situ conservation of medicinal plants in Andhra Pradesh. Proceedings of the National Seminar on Conservation of Eastern Ghats. march 24-26. 2002. 34-54.