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Paper Type | : | Research Paper |
Title | : | "Medicinal Plants from the Landfill Site of Doon Valley" |
Country | : | India |
Authors | : | Seema Manwal, Dr. S.P. Joshi |
: | 10.9790/3008-0523642 |
Keywords-Phytoremediation, solid waste, Nagar Nigam, Landfill, MSW
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Keywords-CCl4, N. sativa, Biochemical Assay, Testis, Wistar Rats
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Paper Type | : | Research Paper |
Title | : | Threat Assessment Of Medicinal Plants Of Koria District In Chhattisgarh (India). |
Country | : | India |
Authors | : | Mantosh Kumar Sinha |
: | 10.9790/3008-0527986 |
Keywords-Threat assessment,Koria district, Medicinal plants
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Abstract : Eighteen samples of vegetable salads sold from three restaurants located at Okada town, Edo State were collected and their bacteriological attributes were investigated using routine methods. The mean heterotrophic and coliform counts recorded for the salad samples ranged from 1.46×104 to 2.80 ×104 CFU/g, and 1.46 ×104 to 2.84 ×104 CFU/g for food centre A. THC and TCC counts (1.74 ×104 to 2.36 ×104 CFU/g and 1.36 ×104 to 2.10 ×104 CFU/g, respectively were obtained for vegetable salads collected from Eatery B. Microbial counts for salads obtained from food service center C varied from 2.08×104 to 2.60 ×104 CFU/g and 1.12 ×104 to 2.90 ×104 CFU/g for THC and TCC, respectively. The differences between the mean bacterial counts were statistically insignificant (P>0.01). Twelve non hemolytic bacterial isolates were identified; Acinetobacter sp., Bacillus sp., Pseudomonas aeruginosa, Proteus mirabilis, Serratia marcescens, Staphylococcus epidermidis, Micrococcus leutus, Enterobacter aerogenes, Escherichia coli, Citrobacter freundii, Klebsiella pneumoniae and Klebsiella oxytoca. E. aerogenes had the highest percentage of occurrence (56%) amongst the isolates. It is recommended that sellers and food handlers within the respective restaurants should make conscious efforts to decontaminate and properly handle the vegetables prior to its salad preparation.
Key words: Coliforms Heterotrophic, Restaurants, Vegetable Salads
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Keywords-Breast cancer, Her-2/neu, IGF-1, L-carnitine, Tamoxifen
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Abstract : A chitinase gene from the thermophilic fungus, Thermomyces lanuginosus was amplified from the genomic DNA of the fungus, by PCR technique. The PCR product was cloned into pXcmkn12 vector by TA cloning technique and sequenced. The gene contained five introns and six exons. The deduced protein sequence coded for 390 amino acids and exhibited very high degree of similarity to other endochitinases of mesophilic and thermophilic fungi. The highly conserved catalytic motif, DGLDIDWEYP, suggests that it belongs to family 18 chitinases.
Keywords: Thermophilic fungus, Thermomyces lanuginosus, Family 18 Chitinase, Introns
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Abstract : Croton scabiosus Bedd. (Euphorbiaceae) is a global endemic to Southern Eastern Ghats of Andhra Pradesh, India. Seed pathological problems, poor germination and recurrent fires in the native habitat are the major causes for low regeneration of this species. With an objective to augment the species population in the natural and allied habitats, the present study focus on developing a standard protocol for mass multiplication of the species through embryo culture. Of the various growth regulators employed for embryo culture for the species, 0.5mg/l BAP+ 3.0mg/l IAA has shown the maximum response and produced healthy plantlets.
Key words - Croton scabiosus, embryo culture, mass multiplication, population augmentation.
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Abstract : In this study the production of substitute rennet by a newly isolated thermophilic fungus, Rhizomucor nainitalensis, using optimized solid substrate fermentation system have been explored. The results revealed that enzyme activity is at its highest level at 40˚C, 50 % humidity, inoculation volume of 103 spore /gr wheat bran, and exoenzyme biosynthesis induction by casein at a rate of 1.5 %. Enzyme activity reached its highest (8400 SU/gr wheat bran) after 110 hours of incubation under the stated conditions. Precipitated enzyme, by addition of ammonium sulfate with milk coagulating activity of 115000 SU/gr enzyme powder, was free of toxin and microorganisms, with market's favorable aroma.
Keywords- Rhizomucor, Solid Substrate Fermentation, rennin, fungal proteases
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