Abstract: Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Rosuvastatin Calcium, Alfuzosine hydrochloride, Atorvastatin Calcium, Ibandronate sodium, Imatinib Mesylate based on their reactivity towards Chloramine-T. The method of each drug depends upon oxidation of drugs by Chloramine-T (Excess) and estimating the amount of unreacted Chloramine-T by Rhodamine-B dye at λmax 557nm. These methods have been applied for the determination of drugs in their pure form as well as in tablet formulations. The effect of excipients has also been studied and found to have no effect. These methods have been validated in terms of guidelines of ICH.............
Keywords: Spectrophotometry, Drugs, Chloramine-T, Rhodamine-B, Quantification, Validation
[1]. Kaila HO, Ambasana MA, Thakkar RS, Saravaia HT, Shan AK. A New improved RP-HPLC Method for assay of rosuvastatin calcium in tablets. Indian J of Pharmaceutical Sciences 2010; 72: 592-598.
[2]. S.C. Sweetman Martindale-The Complete Drug Reference (thirty-sixth ed.), 1The Pharmaceutical Press, London, UK (2009) pp. 1218–1219, 1238–1239, 1284–12851310, 1307–1310.
[3]. Trivedi R.K. Kallem R.R. Mullangi R. Srinivas N.R., Simultaneous determination of rosuvastatin and fenofibric acid in human plasma by LC–MS/MS with electrospray ionization: Assay development, validation and application to a clinical study, J Pharma Biomed Anal., 2005, 39, 661-669.
[4]. Xu D.H. Ruan Z.R. Zhou Q. Yuan H. Jiang B., Quantitative determination of rosuvastatin in human plasma by liquid chromatography with electrospray ionization tandem mass spectrometry, Rapid Commun Mass Spectrum., 2006, 20, 2369-2375.
[5]. Kallem R.R. Karthik A. Chakradhar L. Mullangi R. Srinivas N.R., Development and validation of a highly sensitive and robust LC-MS/MS with electrospray ionization method for quantification of rosuvastatin in small volume human plasma samples and its application to a clinical study, Arzneimittel forsch., 2007, 57, 705-11