IOSR-JAVS   Volume-1 ~ Issue-4 ~ NOV-DEC-2012

ABSTRACT:Speed, heart rate, lactate and uric acid alterations led to reduced performance and hamper the health status of endurance horses. The aim of this study was to investigate on the effects of speed, heart rate, lactate and uric acid on the performance of Arabian horses during a 120 km endurance race. One hundred and eighty four Arabian endurance horses were physically examined and blood samples were collected post-race. After physical examination, the metabolic disordered (MD; n=130) and successfully completed (SC; n = 54) endurance horses were recognized. T-test was used for the analysis. The mean values of speed, heart rate, lactate and uric acid were significantly different (P<0.0001) between the MD and SC endurance horses. In conclusion, alterations in speed, heart rate, blood lactate and uric acid led to poor performance and encumbered the health status of endurance horses. Speed, heart rate, blood lactate and uric acid could be used to appraise performance and health status in endurance horses during training and endurance events.
Key words: endurance horses, heart rate, lactate, speed, uric acid

[1] N. Annamaria, J. D. Sue, and K. M. Jane, A veterinary review of endurance riding as an international competitive sport. Vet J 2012,
1-6.
[2] F. Cottin, N. Metayer, A. G. Goachet, V. Julliand, J. Slawinsk, V. Billat, and E. Barrey, Oxygen consumption and gait variables of
Arabian endurance horses measured during a field exercise test. Equine vet. J 42, 2010, 1-5 doi: 10.1111/j.2042-3306.2010.00184.x.
[3] A. Nagy, J. K. Murray, and S. Dyson, Elimination from elite endurance rides in nine countries: A preliminary study. Equine vet J
42, (38), 2010, 637-643 doi: 10.1111/j.2042-3306.2010.00220.x
[4] Ricard, and Touvais, Genetic parameters of performance traits in horse endurance races. Livestock Science 110, 2007, 118–125.
[5] D. J. Marlin, J. McEwen, and F. Sluyter, Completion and treatment rates in modern endurance racing. In: Proceedings of 4th
International Equitation Science Conference, Dublin, Ireland 2008, 67.
[6] J. Whiting, The exhausted horse. In: Current Therapy in Equine Medicine, Sixth Ed. Saunders Elsevier, St. Louis, MO, USA, 2009,
926–929.
[7] http://www.fei.org/disciplines/endurance/rules
[8] D.H. Lee, and D.R. Jacobs, Association between serum gamma glutamyltransferase and C-reactive protein, Atherosclerosis 178,
2005, 327–330.
[9] F. Fazio, S. Casella, C. Giannetto, G. Caola, and G. Piccione, Serum homocysteine and oxidative stress evaluation during exercise
in horse. Pol J Vet Sci 12, 2007, 169-174.
[10] G. Piccione, F. Fazio, C. Giannetto, A. Assenza, and G. Cao La, Oxidative stress in thoroughbreds during official 1800-metre races.
Veterinarski Arch 2007, 219-227.

ABSTRACT:In vitro multiplication of Rough Lemon (Citrus jambhiri Lush.) was carried out from nodal segments to standardize the protocol for mass multiplication under lab conditions. It was found during experimentation that MS medium supplemented with BAP (1.5 mg/L) and malt extract 500 mg/L resulted in maximum culture establishment, number of shoots and length of longest shoot per culture in minimum time during shoot proliferation. The in vitro multiplied shoots could be best rooted in half strength medium supplemented with IBA and NAA (1.0 mg/L) each with 3 % sucrose as carbohydrate source. In vitro formed plantlets were hardened in potting mixture containing sand, soil and FYM (1:1:1) and highest survival (83.33 %) was achieved after transplantation when rooted plantlet leaves were treated with 50 % glycerol as an antitranspirant.
Key words: Citrus jambhiri, Nodal segments, BAP, Malt extract, Shoot proliferation

[1] Vij, V.K. and H. Kumar, 1990. Citrus rootstock selection, raising and performance in Citriculture in North-Western India. Edited by Gill K.S, Kanwar J.S. & Singh R. (Punjab Agricultural University, Ludhiana, India), 126.

[2] Edriss, M.H. and D.W. Burger, 1984. In vitro propagation of 'Troyer' citrange from epicotyl segments. Scientia Hortic., 23: 159.

[3] Ali, Shawkat and Bushra Mirza (2006) Micropropagation of rough lemon (Citrus jambhiri Lush.): Effect of explants type and hormone concentration. Acta Bot. Croat., 65(2): 137-146.

[4] Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant, 15:473.

[5] Kochaba, J. and P. Spiegel-Roy, 1973. Effect of culture media on embryoid formation from ovular callus of 'Shamouti' orange. Zeit. Fur Pflanz., 69: 159.

[6] Moore, G.A. 1985. Factor affecting in vitro embryogenesis from undeveloped ovules of mature citrus fruit. J. Amer. Soc. Hortic. Sci., 110: 66.

[7] Parthasarathy, V.A. and V. Nagaraju, 1994. Note on embryogenesis in ovules of Cleopatra mandarin (C. reticulata). Indian J Hortic., 51: 49.

[8] Das, T., G.C. Mitra and A. Chatterjee, 1995. Micropropagation of Citrus sinensis cv. Mosambi-An important scion. Phytomorphology, 45: 57

[9] Gloria, F.J., M.M. Filho, F– de. A.A. Camargo and L.E.A. Mendes, 2000. Plant Regeneration from protoplast of Brazilian citrus cultivars. Époques Agropecuaria Brasileira, Brasilia, 35: 727–32.

[10] Chandra, A., V. Gupta, P. Burma and D. Pental, 2003. Patterns of morphogenesis from cotyledon explants of Citron (C. medica L.). In Vitro Cell and Dev. Biol., 39: 514–9.