IOSR-JAVS   Volume-1 ~ Issue-1 ~ SEP-OCT-2012

Paper Type	:	Research Paper
Title	:	Analysis of field-aged pheromone lures in cotton field
Country	:	United Kindom
Authors	:	Ibrahim Salihu Koko, Prof. Alan Cork
	:	10.9790/2380-0110107

ABSTRACT: The purpose of the paper is to determine the longevity, half life and the release rates of Pheromone lures from three commercial companies in India for the Spodoptera litura. Pheromone lures was extracted and examine by the use of Gas chromatography (GC). The major component was detected as ZE-9, 12: 14Ac. Lures from Natural Resource Institute (NRI), Basarass, Agriland as well as PCI lure for the use on Spodoptera litura was aged in cotton field in India in various dispensers like polyethylene vials, rubber tubes, and rubber septum. The result of this research work shows that the standard NRI lures had their lure impregnated with 0.89mg as initial dose and 5.63% remaining life at the 6th week. Basarass pheromone company lure had 0.1mg as initial dose and 12.88% at the end of 6th week, PCI company lure had -0.01mg as initial dose and 7.84% remaining life at the end of the 6th week, Agriland company lure was impregnated with 0.05mg as initial dose and 48.02% remaining life at 6th week respectively. The conclusion that was drawn is that companies like Basarass and Agriland do not put enough milligram (mg) of the major component and the release rates fluctuates on the field due to impurities and this had given the cure some measure of longevity with adverse effect on the performance. Among the recommendations that have been made in this paper companies should endeavor to increase the amount of milligrams impregnated in the lure and find the means and ways of increasing longevity without compromising on the fast release of the major component.
Key words: Pheromone, lures, cotton

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Abstract : In the present study, rabies was confirmed by Nested RT-PCR on saliva of 24 animals suspected to be rabid. Amplification with first round primers (Rab N1 and Rab N5) yielded a 1477 bp product, while primers used for second round amplification (RabNfor and RabNrev) yielded a 762 bp product. By nested RT-PCR, viral RNA could be detected in 9/24 (36.0%) saliva samples. Confirmatory diagnosis by Immunofluorescence performed on brain sample revealed 17 true positive cases. Sensitivity of 68.0% was recorded by application of Nested RT-PCR on saliva samples. It was concluded that Nested RT-PCR applied on saliva of rabies suspected animal can help in intravitam diagnosis of rabies. Keywords: Immunofluorescence, Intravitam, Nested RT-PCR, Rabies, Saliva.

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